Millwall's Under 23s were defeated 2-0 by Colchester United in the Professional Development League on Monday night.
Kai Redgrave and Tom Stagg found the net for The U's on a night that saw Sean O'Brien's penalty-kick saved by Endurance Johnson with the score goalless.
Ben Thompson played the full 90 minutes for Kevin Nugent's side.
Next up for the Under 23s is a trip to Ipswich Town on Monday 22nd November.
Millwall: Wright, Walker, Garande, Allen, Okoli, Topalloj, Boateng, Thompson, Briscoe, O’Brien (Leahy 85'), Drozd (Abdulmalik 71'). Subs not used: Gillmore, Smith.