Millwall's Under 23s were defeated 3-0 by Bristol City in the Professional Development League on Friday night.
Kevin Nugent's side followed up on last Monday's 1-1 draw against Cardiff City with a loss against The Robins, who sealed all three courtesy of goals from Jake Andrews, Arnie Garita and Connor Lemonheigh-Evans.
Next up for Millwall is a trip to the Ricoh Arena to play Coventry City on Monday 30th October.
Millwall: Sandford, McNamara, Chesmain, Hanson, Mbulu, Rooney, Olaofe (Debrah 83'), Wicks, Brymora (Mitchell 75'), White, Saunders-Henry. Sub not used: Jackson.