Millwall's Under 23s suffered a rare Professional Development League defeat on Tuesday afternoon as a strong Wigan Athletic side were 2-0 winners at Calmont Road.
James Carragher gave The Latics the lead on the stroke of half-time, with Charlie Jolley notching a second as the clock struck 80 minutes.
The Lions had chances of their own, but hit the crossbar through Besart Topalloj and had a George Alexander penalty saved.
Reuben Duncan returned from his loan spell at Welling United to take his place in the starting XI, whilst Frank Fielding continued his quest to find match fitness in goal for Kevin Nugent's side.
The pace of the game and the chances in front goal were fast and frenetic in the opening stages, but Alexander and Hayden Muller were unable to convert, whilst at the other end, Jolley and Thelo Aasgaard tried their luck to no avail.
Alexander and Duncan saw efforts stopped by Bobby Jones around the midway point of the first-half, with Aasgaard again in amongst it at the summit of Wigan's attacking efforts, but he struck wide of the post.
The Latics' number 10 then wasted the best opportunity to date on 32 minutes, as - after Muller dallied on the ball - he raced away in a one-on-one situation with Fielding, but blazed his effort well over the crossbar.
On the stroke of half-time, indecision in The Lions' defence allowed Wigan to take advantage and square for the awaiting Carragher, who had the easiest of tasks to fire into an empty net and give The Latics the lead.
Millwall, however, came back out for the second-half with a purpose and were unlucky not to level the game - or more - early on. Moss shot over, before Besart Topalloj's strike from inside the penalty area cracked the woodwork and bounced to safety.
Just two minutes later, The Lions were awarded a spot-kick after Alexander was brought down by goalkeeper Bobby Jones, but after the striker picked himself up to take the penalty, his kick was well saved by the stopper.
Moss and Aasgaard traded efforts at either end as both sides probed for more goalmouth action, but despite Kevin Nugent's side having large spells of possession, the killer blow came 10 minutes from time as Jolley turned home at the back post.
Next up for Millwall's Under 23s is another home game - this time against Cardiff City - next Wednesday morning.
Millwall: Fielding, J. Brown (A. Mitchell 46'), Topalloj (O'Brien 85'), Moss, Muller, Strachan, Skeffington, B. Mitchell (Boateng 46'), Alexander, Duncan, Davis. Sub not used: Gillmore.
Wigan Athletic: Jones, Lloyd, Webber, Carragher, Long, McHugh, Banincime, McGee, Jolley, Aasgaard, O'Neil. Subs: Mooney, Hughes, M. Brown, Aderko, McGurk.
Scorer: Carragher 45', Jolley 80'
Booked: McHugh, Lloyd
Referee: Phillip Stayings