Neil Harris admits he wants to send a statement to Sky Bet League One rivals when Bury travel to The Den on Saturday afternoon.
Millwall crashed out of the Emirates FA Cup last weekend, ending a 17 match unbeaten run across all competitions.
The rare defeat has sparked calls for a "strong reaction" from players as the number of remaining games approaches single figures.
"It's an opportunity for us to show that we will and can continue our fine League form," Harris told millwallfc.co.uk
"We are still unbeaten in the League since mid-December and have a chance to keep the pressure on the top teams in the division this weekend."
The Lions have a taxing run of away matches ahead of them, including trips to League leaders Sheffield United and Fleetwood, and Harris has highlighted the importance of fan support.
"Our supporters have been fantastic, they have really helped to give us the edge and there is a special bond between the club and fans," Harris added.
"I want to remind them that we only have four home games left and we really need the crowd to get behind us in those matches, starting against Bury on Saturday."
Chopper may be forced to shuffle his squad with Steve Morison and recovering goalkeeper Jordan Archer a '50/50' chance to be available selection.
"Jordan Archer is back out training but it's just whether we can get his kicking back up to speed. That's the issue with his hip-flexor injury, it will test if he can kick the volume of balls that he needs to on Saturday.
"Steve came off as a precaution against Spurs at the weekend, but he has had a slight niggle in his calf before. We will make a late decision in if he will be involved this weekend."